Translocation of 3H-DNA, 131I-ribonuclease and 3H-DNA 131I-ribonuclease complexes in germinated barley grains
Creators
- 1. Centre nucléaire TRICO, Kinshasa (Congo, The Democratic Republic of the)
- 2. Centre d'étude de l'énergie nucléaire Mol (Belgium)
Description
Barley grains, after germinating for 11 hours in the presence of water, were cut into sections at the end opposite the embryo. They were incubated in solutions of 3H-DNA, 13II-ribonuclease, and 3H-DNA 131I-ribonuclease complex for three hours. They were then placed in a water-saturated atmosphere for 24 hours. At this stage the different organs of the seedlings were separated and homogenized in a solution containing 0.15M sodium chloride and 0.1 M sodium ethylenediaminetetraacetate at pH 7. By measuring the radioactivity found in the homogenates one can estimate the penetration of the macromolecules under study. The results show that the quantity found varies from one case to the other and depends both on the nature of the macromolecule and of the organ studied. (author)
Abstract (French)
Des orges qui ont germé pendant 11 h en présence d’eau sont sectionnées au bout opposé à l’embryon. Elles sont incubées avec des solutions d’ADN3H, de RNase1311 et de complexe ADN3H - RNase 131I, pendant 3 h. Elles sont ensuite placées dans une atmosphère saturée d'eau pendant 24 h. A ce moment, les différents organes des plantules sont séparés et homogénéisés en présence d'une solution 0,15M en NaCl et 0,1M en éthylènediamine-tétracétate de Na à pH 7. La mesure de la radioactivité retrouvée dans les homogénats permet d'évaluer la pénétration des macromolécules considérées. Les résultats montrent que la quantité retrouvée varie d'un cas â l'autre et dépend à la fois de la nature de la macromolécule et de l'organe considéré. (author)Additional details
Additional titles
- Original title (French)
- Translocation des ADN3H, RNase131I et complexes ADN3H - RNase 131I dans les orges en germination
Publishing Information
- Publisher
- IAEA
- Imprint Place
- Vienna (International Atomic Energy Agency (IAEA))
- Imprint Title
- Peaceful uses of atomic energy in Africa. Proceedings of a symposium
- Imprint Pagination
- 595 p.
- Series
- Proceedings Series
- Journal Page Range
- p. 297-304
- ISSN
- 0074-1884
Conference
- Title
- Symposium on the peaceful uses of atomic energy in Africa
- Dates
- 28 Jul - 1 Aug 1969
- Place
- Kinshasa (Congo, The Democratic Republic of the)
INIS
- Country of Publication
- International Atomic Energy Agency (IAEA)
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46129852
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- BARLEY; DNA; EMBRYOS; HOMOGENATES; IODINE 131; PH VALUE; RNA-ASE; SEEDLINGS; SODIUM; SODIUM CHLORIDES; SOLUTIONS; TRITIUM; WATER
- Descriptors DEC
- ALKALI METAL COMPOUNDS; ALKALI METALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CEREALS; CHLORIDES; CHLORINE COMPOUNDS; DAYS LIVING RADIOISOTOPES; DISPERSIONS; ELEMENTS; ENZYMES; ESTERASES; GRAMINEAE; HALIDES; HALOGEN COMPOUNDS; HOMOGENEOUS MIXTURES; HYDROGEN COMPOUNDS; HYDROGEN ISOTOPES; HYDROLASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; LIGHT NUCLEI; LILIOPSIDA; MAGNOLIOPHYTA; METALS; MIXTURES; NUCLEASES; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHODIESTERASES; PLANTS; PROTEINS; RADIOISOTOPES; SODIUM COMPOUNDS; SODIUM HALIDES; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- Refs., 2 figs., 5 tabs. Imprint:Utilisation de l'#Latin Small Letter E With Acute#nergie atomique #Latin Small Letter A With Grave# des fins pacifiques en Afrique. Compte rendu d'un colloque
- Secondary number(s)
- STI/PUB--233