Effects of DNA damage in peripheral blood lymphocytes of human induced by γ-rays irradiation

Objective: To investigate the dose-effect curve of the human Peripheral Blood Lymphocytes irradiated by γ-rays. Methods: The immediate irradiation-caused DNA damage in the human Peripheral Blood Lymphocytes irradiated with γ-rays ws investigated. The single cell gel electrophoresis (SCGE, Comet Assay) were used to detect the extent of DNA damage. Results: After 0 Gy, 0.1 Gy, 0.5 Gy, 1 Gy, 2 Gy and 3 Gy γ-rays irradiation, the curve of dose(x) to content of DNA in tail(y) is y=-1.1063x2 + 10.398x+3.5362, while the curve of dose(x) to tail moment(y) is y=-0.0345x2 + 3.5282x + 0.6542. Conclusion: The results indicated that the content of DNA in tail and tail moment for the immediate DNA damage caused by irradiation had a positively binomial curve correlation with the irradiation dose in the dose range of 0-3 Gy, and SCGE might be used as a new biological dosimeter. (authors)