Published 1977 | Version v1
Journal article Metadata-only

DNA synthesis during development and proliferation of glial cells in organotypic rat cerebellar culture

Creators

  • 1. Polska Akademia Nauk, Warsaw. Centrum Medycyny Doswiadczalnej i Klinicznej

Description

DNA synthesis was investigated in glial cells in vitro with 3H thymidine in concentration 1 μCi/ml medium. Incorporation of isotope into the glial nuclei has been found both in the explant (7-21%) and in the outgrowth (22-56%). DNA synthesis was dependent on the age of culture and due to the contact inhibition in the outgrowth. Results point out that marked DNA synthesis is a characteristic feature of glia differentiation and of reactive character of glial cells in vitro. (author)

Additional details

Additional titles

Original title (Polish)
Synteza DNA w procesie dojrzewania i wzrostu komorek glejowych w hodowli organotypowej mozdzku szczura in vitro

Publishing Information

Journal Title
Neuropatol. Pol.
Journal Volume
15
Journal Issue
3
Series
Neuropatol. Pol.
Journal Page Range
341-348

INIS

Country of Publication
Poland
Country of Input or Organization
Poland
INIS RN
10484603
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
AGE DEPENDENCE; AUTORADIOGRAPHY; BIOSYNTHESIS; CELL DIFFERENTIATION; CEREBELLUM; DNA; GROWTH; IMAGES; IN VITRO; RATS; THYMIDINE; TISSUE CULTURES; TRITIUM COMPOUNDS
Descriptors DEC
ANIMALS; AZINES; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; MAMMALS; NERVOUS SYSTEM; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RIBOSIDES; RODENTS; SYNTHESIS; VERTEBRATES