Published April 2, 1979 | Version v1
Journal article Metadata-only

The preparation of 125I-labelled bile acid ligands for use in the radioimmunoassay of bile acids

Creators

  • 1. Edinburgh Univ. (UK). Royal Infirmary
  • 2. Medical Research Council, Edinburgh (UK)

Description

A general method for the preparation of 125I-labelled bile acid-histamine or 125I-labelled bile acid-tyramine conjugates is presented. The method is simple, quick and produces ligands in good yield (30%). The characteristics of a radioimmunoassay for conjugated chenodeoxycholic acid, based on an 125I-labelled ligand prepared by the method, are also described. The assay produces values for fasting serum concentrations of conjugated chenodeoxycholic acid that agree well with previous data. (Auth.)

Additional details

Publishing Information

Journal Title
Clin. Chim. Acta
Journal Volume
93
Journal Issue
1
Series
Clin. Chim. Acta.
Journal Page Range
145-150
ISSN
0009-8981

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
10468901
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
BILE ACIDS; BLOOD SERUM; HISTAMINE; IODINE 125; LABELLING; LIGANDS; RADIOIMMUNOASSAY; TYRAMINE
Descriptors DEC
AMINES; AROMATICS; AZOLES; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IMIDAZOLES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHENOLS; RADIOISOTOPES; STEROIDS; STEROLS; SYMPATHOMIMETICS; TRACER TECHNIQUES