Rapid method for the preparation of 125I-labelled human growth hormone for receptor studies, using reverse-phase high performance liquid chromatography
Creators
- 1. Univ. of Leuven School of Medicine, A.Z. Gasthuisberg, Belgium
Description
Human growth hormone was labelled with 125 Iodine by the stoichiometric modification of the chloramine-T method to a specific activity of 50-80 microCi/microgram, and the iodinated mixture was purified by reverse-phase high performance liquid chromatography using a C18 column (SynChropak RP-P) and a linear gradient. Compared with the usual Sephadex G-100 chromatography, HPLC gave a much better separation, with a higher yield and a considerably reduced analysis time (30 min vs 5 h). The [125I]-labelled preparation had normal binding to IM-9 lymphocyte receptors. The maximum bindability of the HPLC-purified preparation approximated 90%, which is the best value so far reported for human growth hormone. It is concluded that HPLC is a fast, convenient and reproducible method for obtaining an improved [125I]-labelled human growth hormone for receptor studies
Additional details
Publishing Information
- Journal Title
- Biochem. Biophys. Res. Commun.
- Journal Issue
- no.2
- Series
- Biochem. Biophys. Res. Commun.
- ISSN
- 0006-291X
- CODEN
- BBRCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 17077882
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- CHEMICAL PREPARATION; IODINE 125; LABELLED COMPOUNDS; LABELLING; LIQUID COLUMN CHROMATOGRAPHY; RADIOCHEMISTRY; RECEPTORS; STH
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; CHEMISTRY; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOISOTOPES; SEPARATION PROCESSES; SYNTHESIS