Published June 2000
| Version v1
Journal article
Participation of ATM in cellular response to DNA damage induced by ionizing radiation
- 1. Department of Biochemistry, Inst. of Radiation Medicine, Beijing (China)
Description
Objective: To clone ATM full length cDNA and cDNA fragments containing some functional domains and to identify proteins that interact with ATM and mediate DNA damage signal transduction in cellular response to DNA damage. Methods: ATM cDNA was amplified from MarthomTM-Ready cDNA kit of human leukocytes by LD-PCR. ATM-interacting proteins were screened by yeast two hybrid system. Results: ATM full-length cDNA and cDNA fragments containing PI3K kinase domain, leucine zipper and proline rich region were amplified from human cDNAs. Several candidate clones that interacted with ATM PI3K domain were identified. Conclusion: ATM mediates DNA damage signal transduction by interacting with many proteins
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 20
- Journal Issue
- 3
- Journal Page Range
- p. 149-152
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 32012024
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CLONING; CYTOLOGY; DNA; GENES; HYBRIDIZATION; MOLECULAR BIOLOGY; PROTEINS; RADIATION INJURIES; YEASTS
- Descriptors DEC
- BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGY; DISEASES; EUMYCOTA; FUNGI; INJURIES; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; RADIATION EFFECTS