Complementary LEEM and eV-TEM for imaging and spectroscopy

Highlights: • LEEM and eV-TEM imaging of the same area at 0-75 eV • 3 eV Transmission Electron Microscopy with sub 10 nanometer resolution • Graphene and graphene oxide membranes used as support films for sample preparation from solution • Prolonged imaging of DNA origami without visible degradation Transmission electron microscopy at very low energy is a promising way to avoid damaging delicate biological samples with the incident electrons, a known problem in conventional transmission electron microscopy. For imaging in the 0-30 eV range, we added a second electron source to a low energy electron microscopy (LEEM) setup, enabling imaging and spectroscopy in both transmission and reflection mode at nanometer (nm) resolution. The latter is experimentally demonstrated for free-standing graphene. Exemplary eV-TEM micrographs of gold nanoparticles suspended on graphene and of DNA origami rectangles on graphene oxide further establish the capabilities of the technique. The long and short axes of the DNA origami rectangles are discernable even after an hour of illumination with low energy electrons. In combination with recent developments in 2D membranes, allowing for versatile sample preparation, eV-TEM is paving the way to damage-free imaging of biological samples at nm resolution.