Selective 3,4-Dihydroxyphenylalanine Analysis in Human Urine as Ethoxycarbonyltert- butyldimethylsilyl Derivatives by Gas Chromatography-Mass Spectrometry
Creators
- 1. Ajou University, Suwon (Korea, Republic of)
- 2. Sungkyunkwan University, Suwon (Korea, Republic of)
Description
A new analytical method for measurement of 3,4-dihydroxyphenylalanine (DOPA) in human urine was developed. DOPA from an aqueous solution was converted into an ethoxycarbonyl (EOC) derivative. A tertbutyldimethylsilyl (TBDMS) reaction under anhydrous conditions was then attempted for analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. A new mass spectral data on DOPA as a tri-EOC/mono-TBDMS derivative was built. This method showed good linearity (r ≥ 0.999), precision (% relative standard deviation = 3.1-9.2), and accuracy (% relative error = .7.2-8.8), with a detection limit of 0.05 ng/mL. This selective and accurate method of DOPA analysis will be useful for biochemical monitoring of various neurological disorders including Parkinson's disease in biological fluids
Additional details
Publishing Information
- Journal Title
- Bulletin of the Korean Chemical Society
- Journal Volume
- 32
- Journal Issue
- 3
- Series
- 26 refs, 3 figs, 1 tab
- Journal Page Range
- p. 977-980
- ISSN
- 0253-2964
INIS
- Country of Publication
- Korea, Republic of
- Country of Input or Organization
- Korea, Republic of
- INIS RN
- 48087178
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- ACCURACY; AQUEOUS SOLUTIONS; CHROMATOGRAPHY; DOPA; MASS SPECTROSCOPY; MONITORING; NEUROLOGY; SPECTRA; URINE
- Descriptors DEC
- AMINO ACIDS; AUTONOMIC NERVOUS SYSTEM AGENTS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CARBOXYLIC ACIDS; DISPERSIONS; DRUGS; HOMOGENEOUS MIXTURES; HYDROXY ACIDS; MATERIALS; MEDICINE; MIXTURES; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; SEPARATION PROCESSES; SOLUTIONS; SPECTROSCOPY; WASTES