Published February 2013
| Version v1
Journal article
Immunochromatographic lateral flow strip for on-site detection of bisphenol A
Creators
- 1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009 (China)
- 2. School of Food Science and Technology, Jiangnan University, Wuxi, 210004 (China)
- 3. Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing, 200002 (China)
Description
We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results. (author)
Additional details
Identifiers
Publishing Information
- Journal Title
- Microchimica Acta (Online)
- Journal Volume
- 180
- Journal Issue
- 3-4
- Journal Page Range
- p. 279-285
- ISSN
- 1436-5073
INIS
- Country of Publication
- Austria
- Country of Input or Organization
- Austria
- INIS RN
- 44052688
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY; S54: ENVIRONMENTAL SCIENCES;
- Descriptors DEI
- ANTIBODIES; BUFFERS; COMPUTER CODES; FOOD; GOLD; NANOSTRUCTURES; PARTICLES; PH VALUE; PROBES; SAFETY; SENSITIVITY; SPECIFICITY; WATER
- Descriptors DEC
- ELEMENTS; HYDROGEN COMPOUNDS; METALS; OXYGEN COMPOUNDS; TRANSITION ELEMENTS