Mechanisms for carbon dots-based chemosensing, biosensing, and bioimaging: A review

Highlights: • Fluorescence sensing mechanisms for CDs probes have been overviewed. • Recent progress on CDs probes with various sensing mechanisms are summarized. • Future prospective of other sensing mechanisms for CDs probes are discussed. Due to the favorable biocompatibility, photostability and fluorescence emissions, carbon dots (CDs) are being widely investigated as fluorescent probes. Current CD-based fluorescent probe designs depend largely on conventional fluorescence sensing mechanisms, for e.g. the inner filter effect, photoinduced electron transfer, and Förster resonance energy transfer. Although these mechanisms have been successful, it is still desirable to introduce new sensing mechanisms. In recent years, emerging mechanisms such as aggregation-induced emission, hydrogen-bond induced emission, and intramolecular charge transfer have been developed for CD-based probes. This review summarizes both conventional and emerging mechanisms, and discuss CDs in the context of chemosensing, biosensing, and bioimaging. We provide an outlook for several other mechanisms such as CN isomerization, the short-wavelength inner filter technique, excited-state intramolecular proton transfer, and twisted intramolecular charge transfer, which have been applied to organic fluorescent probes design but not as much in CD-based sensing systems. We envision that this review will provide insights that inspire further development of CD-based fluorescent probes as for biological applications.