Published March 2006 | Version v1
Journal article Metadata-only

Effects of DNA damage in peripheral blood lymphocytes of human induced by γ-ray irradiation

Creators

  • 1. Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang (China)
  • 2. School of Live Sciences, Lanzhou Uinv., Lanzhou (China)

Description

The immediate irradiation-caused DNA damage in Peripheral Blood Lymphocytes of human irradiated with γ-rays was investigated, and the single cell gel electrophoresis(SCGE, Comet Assay) was used to detect the DNA damage. After 0Gy, 0.1 Gy, 0.5Gy, 1 Gy, 2Gy, 3Gy γ-rays irradiation, content of DNA in tail is 4.66%, 5.22%, 5.74%, 12.75%, 21.59% and 24.11%, while tail moment is 1.02, 0.95, 1.24, 5.40, 7.06 and 13.05. The results indicated that the content of DNA in tail and tail moment for the immediate irradiation-cased DNA damage had a positively linear correlation with the irradiation dose in the dose range of 0-3 Gy, and SCGE can be to serve as a new biological dosimeter. (authors)

Additional details

Publishing Information

Journal Title
Nuclear Science and Technology Applications
Journal Volume
2
Journal Issue
1
Journal Page Range
p. 34-37
CODEN
HJYYAS

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
42016682
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL DOSEMETERS; DNA DAMAGES; DOSE-RESPONSE RELATIONSHIPS; ELECTROPHORESIS; GAMMA RADIATION; GELS; HUMAN POPULATIONS; IRRADIATION; LYMPHOCYTES; RADIATION DOSES; RADIATION INJURIES
Descriptors DEC
ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; COLLOIDS; CONNECTIVE TISSUE CELLS; DISEASES; DISPERSIONS; DOSEMETERS; DOSES; ELECTROMAGNETIC RADIATION; INJURIES; IONIZING RADIATIONS; LEUKOCYTES; MATERIALS; MEASURING INSTRUMENTS; POPULATIONS; RADIATION EFFECTS; RADIATIONS; SOMATIC CELLS

Optional Information

Notes
3 figs., 1 tab., 11 refs.