Published April 20, 2007 | Version v1
Journal article Metadata-only

Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase from Rhodopseudomonas palustris CGA009

Creators

  • 1. Laboratory of Structural Biology, School of Medicine, Tsinghua University, Beijing 100084 (China)
  • 2. Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford OX1 3QR (United Kingdom)
  • 3. National Laboratory of Biomacromolecules, Institute of Biophysics (IBP), Chinese Academy of Sciences, Beijing 100101 (China)

Description

Palustrisredoxin reductase (RPA3782, PuR), a flavin-dependent ferredoxin reductase, is an essential component of the Class I cytochrome P450 systems in Rhodopseudomonas palustris CGA009. Crystals of PuR that diffract to 2.2 Å resolution have been obtained. Palustrisredoxin reductase from Rhodopseudomonas palustris CGA009, a member of the oxygenase-coupled NADH-dependent ferredoxin reductase (ONFR) family, catalyzes electron transfer from NADH to ferredoxins. It is an essential component of the cytochrome P450 systems in R. palustris CGA009, a model organism with diverse metabolic pathways. Here, the crystallization of palustrisredoxin reductase is reported. The crystals belong to the trigonal space group P3221, with unit-cell parameters a = 107.5, b = 107.5, c = 69.9 Å, and diffract to 2.2 Å resolution on a synchrotron source

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309107017411; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335014

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335014;
DOI
10.1107/S1744309107017411;
PII
S1744309107017411;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
63
Journal Issue
Pt 5
Journal Page Range
p. 422-425
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46065614
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; ELECTRON TRANSFER; OXYGEN; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION
Descriptors DEC
COHERENT SCATTERING; DIFFRACTION; ELEMENTS; NONMETALS; PHASE TRANSFORMATIONS; SCATTERING; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2007
Notes
PMCID: PMC2335014; PMID: 17565187; PUBLISHER-ID: pu5184; OAI: oai:pubmedcentral.nih.gov:2335014