Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc
Description
Photoaffinity labeling of purified cellulose synthase with [beta-32P]5-azidouridine 5'-diphosphoglucose (UDP-Glc) has been used to identify the UDP-Glc binding subunit of the cellulose synthase from Acetobacter xylinum strain ATCC 53582. The results showed exclusive labeling of an 83-kDa polypeptide. Photoinsertion of [beta-32P]5-azido-UDP-Glc is stimulated by the cellulose synthase activator, bis-(3'----5') cyclic diguanylic acid. Addition of increasing amounts of UDP-Glc prevents photolabeling of the 83-kDa polypeptide. The reversible and photocatalyzed binding of this photoprobe also showed saturation kinetics. These studies demonstrate that the 83-kDa polypeptide is the catalytic subunit of the cellulose synthase in A. xylinum strain ATCC 53582
Additional details
Publishing Information
- Journal Title
- Journal of Biological Chemistry
- Journal Volume
- 265
- Journal Issue
- 9
- Series
- J. Biol. Chem.
- Journal Page Range
- 4782-4784
- ISSN
- 0021-9258
- CODEN
- JBCHA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21067387
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AFFINITY; AUTORADIOGRAPHY; BACTERIA; CELL MEMBRANES; ENZYME ACTIVITY; GLYCOSYL TRANSFERASES; LABELLING; MOLECULAR WEIGHT; PHOSPHORUS 32; POLYPEPTIDES
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; ENZYMES; ISOTOPES; LIGHT NUCLEI; MEMBRANES; MICROORGANISMS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PEPTIDES; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; TRANSFERASES