Published January 1989
| Version v1
Journal article
Evidence for a single class of somatostatin receptors in ground squirrel cerebral cortex
- 1. Institut for Biological Research Dr S. Stankovic, Beograd (Yugoslavia)
Description
In the present study we characterized high-affinity somatostatin (SRIF) binding sites (Kd = 2.06 +/- 0.32 nM and Bmax = 295 +/- 28 fmol/mg protein) in cerebral cortex membrane preparations of European ground squirrel using 125I-[Tyr0-D-Trp8]-SRIF14 as a radioligand. The inhibition of radioligand specific binding by SRIF14, as well as by its agonists (SRIF28, Tyr0-D-Trp8-SRIF14, SMS 201 995) was complete and monophasic, thus revealing a single population of somatostatinergic binding sites. Radioautographic analysis of 125I-[Tyr0-D-Trp8]-SRIF14 labeled brain sections confirmed the results of our biochemical study. The homogeneity of SRIF binding sites in the ground squirrel neocortex was not dependent on the animal's life-cycle phase
Additional details
Publishing Information
- Journal Title
- Peptides (Fayetteville, New York)
- Journal Volume
- 10
- Journal Issue
- 1
- Series
- Peptides (Fayetteville, N.Y.).
- Journal Page Range
- 57-62
- ISSN
- 0196-9781
- CODEN
- PPTDD
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21006265
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AUTORADIOGRAPHY; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; CEREBRAL CORTEX; HIBERNATION; IODINE 125; LIGANDS; RECEPTORS; SOMATOSTATIN; SQUIRRELS
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BODY; BRAIN; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; CEREBRUM; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPES; KINETICS; MAMMALS; MEMBRANES; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANS; RADIOISOTOPES; REACTION KINETICS; RODENTS; VERTEBRATES