Dual-mode of Reversed and Normal-phase Liquid Chromatographic Enantiomer Separation on Coated Cellulose Derived Chiral Stationary Phases

In summary, we demonstrated enantiomer separation of N-FMOC α-amino acids on coated cellulose derived CSPs, Chiralcel OJ-H and Chiralcel OD-H under both normal and reversed-phase conditions. Although the separation factors for resolution of N-FMOC α-amino acids in reverse mode are generally lower than those in normal mode, these CSPs can be usefully applied in dual mode, not only normal-phase conditions but also reversed-phase conditions for enantiomer resolution of N-FMOC α-amino acids. In particular, this reversed-phase analytical method using Chiralcel OD-H is expected to be quite useful for determination of the enantiomeric purity of analytical samples in biological matrices of serum or plasma and in asymmetric synthesis using aqueous solvents