Published 1997
| Version v1
Journal article
A simplified method for the preparation of α-32P-labeled deoxyribonucleoside triphosphates
Description
A method for preparing α-32P-labeled deoxyribonucleoside triphosphates ([α-32P]dNTP) is described. By comparing the labeling procedure to that of Walseth et al. 1,2 the reaction of Nuclease P1 is saved in our experiments and only three steps are needed for a routine preparation, without decreasing the yield of [α-32P]dNTP which is over 92% based on 32Pi. In this paper [α-32P]dATP is taken as an example to describe the labeling procedure, the suitability of our method has been discussed. (author)
Additional details
Publishing Information
- Journal Title
- Journal of Radioanalytical and Nuclear Chemistry
- Journal Volume
- 218
- Journal Issue
- 1
- Journal Page Range
- p. 93-95.
- ISSN
- 0236-5731
- CODEN
- JRNCDM
INIS
- Country of Publication
- Hungary
- Country of Input or Organization
- Hungary
- INIS RN
- 28046427
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- ATP-ASE; CHEMICAL PREPARATION; CHEMICAL REACTION YIELD; LABELLING; PH VALUE; PHOSPHORUS 32; RADIOACTIVITY; TIME DEPENDENCE
- Descriptors DEC
- ACID ANHYDRASES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ENZYMES; HYDROLASES; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHOHYDROLASES; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; SYNTHESIS; YIELDS
Optional Information
- Notes
- 7 refs.