Effectiveness and efficiency of physical, chemical and physico-chemical mutagens in M2 generation of Vicia faba L. var VH82-1

The mutagenic effectiveness, efficiency and efficacy of gamma rays (10, 20 and 30 Krad), EMS (0.10 and 0.40 %) their combination treatments 10, 20, 30 Krad-gamma rays + 0.10% EMS and 10, 20, 30 Krad gamma rays + water were studied on Vicia faba L var. VH82-1. Effectiveness of mutagens measured by the rate of mutation as related to unit dose of mutagens, was found to be in order of EMS> gamma rays + EMS> gamma rays + water> gamma rays. Efficiency of mutagens as estimated by the rate of mutation in relation to other biological effects induced usually a measure of damage in M1 was gamma rays + water> gamma rays + EMS> gamma rays> EMS (MP/L, MP/S, MP/CA). Gamma rays 20 Krad sole or combined either with EMS or water were found to be most effective and efficient doses and 0.10% EMS was most effective concentration. (author). 10 refs., 2 tabs