Biologically mediated abiotic degradation (BMAD) of bisphenol A by manganese-oxidizing bacteria

Highlights: • MOB mediate enhanced BPA degradation at low (μM range) Mn(II) concentrations. • MOB contribute to BPA degradation, but organism-specific differences exist. • Mn flux determines efficacy of coupled biotic-abiotic BPA degradation. • Oxic-anoxic interfaces are hotspot barriers for BPA degradation. • The BMAD process, both natural and engineered, promises sustainable attenuation of BPA. Bisphenol A (BPA), a chemical of environmental concern, is recalcitrant under anoxic conditions, but is susceptible to oxidative degradation by manganese(IV)-oxide (MnO₂). Microbial Mn(II)-oxidation generates MnO_2-bio; however, BPA degradation in cultures of Mn(II)-oxidizing bacteria has not been explored. We assessed MnO_2-bio-mediated BPA degradation using three Mn(II)-oxidizing bacteria, Roseobacter sp. AzwK-3b, Erythrobacter sp. SD-21, and Pseudomonas putida GB-1. In cultures of all three strains, enhanced BPA degradation was evident in the presence of Mn(II) compared to replicate incubations without Mn(II), suggesting MnO_2-bio mediated BPA degradation. Increased Mn(II) concentrations up to 100 µM resulted in more MnO_2-bio formation but the highest BPA degradation rates were observed with 10 µM Mn(II). Compared to abiotic BPA degradation with 10 μM synthetic MnO₂, live cultures of strain GB-1 amended with 10 μM Mn(II) consumed 9-fold more BPA at about 5-fold higher rates. Growth of strain AzwK-3b was sensitive to BPA and the organism showed increased tolerance against BPA in the presence of Mn(II), suggesting MnO_2-bio alleviated the inhibition by mediating BPA degradation. The findings demonstrate that Mn(II)-oxidizing bacteria contribute to BPA degradation but organism-specific differences exist, and for biologically-mediated-abiotic-degradation (BMAD), Mn-flux, rather than the absolute amount of MnO_2-bio, is the key determinant for oxidation activity.