Evidence of multi-decadal behavior and ecosystem-level changes revealed by reconstructed lifetime stable isotope profiles of baleen whale earplugs

Highlights: • Reconstructed life-history δ¹³C and δ¹⁵N profiles in baleen whale • Evidence of the Suess effect in whale earplugs dating back to the 1930s • Life history behavior changes were observed in δ¹³C and δ¹⁵N profiles. • Long-term ecological change was identified using δ¹³C lifetime profiles. Biological time series datasets provide an unparalleled opportunity to investigate regional and global changes in the marine environment. Baleen whales are long-lived sentinel species and an integral part of the marine ecosystem. Increasing anthropogenic terrestrial and marine activities alter ocean systems, and such alterations could change foraging and feeding behavior of baleen whales. In this study, we analyzed δ¹³C and δ¹⁵N of baleen whale earplugs from three different species (N = 6 earplugs, n = 337 laminae) to reconstruct the first continuous stable isotope profiles with a six-month resolution. Results of our study provide an unprecedented opportunity to assess behavioral as well as ecological changes. Abrupt shifts and temporal variability observed in δ¹³C and δ¹⁵N profiles could be indicative of behavior change such as shift in foraging location and/or trophic level in response to natural or anthropogenic disturbances. Additionally, five out of six individuals demonstrated long-term declining trends in δ¹³C profiles, which could suggest influence of emission of depleted ¹³CO₂ from fossil fuel combustion referred to as the Suess effect. After adjusting the δ¹³C values of earplugs for the estimated Suess effect and re-evaluating δ¹³C profiles, significant decline in δ¹³C values as well as different rate of depletion suggest contribution of other sources that could impact δ¹³C values at the base of the food web.