Steroid uptake and 3H-uridine incorporation by early hamster and squirrel monkey (in vitro fertilized) embryos

Effects of ovulatory regimens were analyzed on the uptake of 3H-estradiol (E2) and 3H-progesterone (P) and incorporation and uptake of 3H-uridine by hamster and squirrel monkey (in vitro fertilized) embryos throughout early preimplantation development. E2 and P uptake increased throughout preimplantation development in both species. The pregnant mare's serum/human chorionic gonadotropin superovulatory regimen reduced uptake of steroids by the hamster embryo at virtually all stages analyzed. There was no effect on the incorporation of 3H-uridine (used as an indicator of relative RNA synthesis). The length of FSH treatment had no appreciable effects on steroid uptake by squirrel monkey embryos fertilized in vitro. (author)