Expression of IL-10, TNF-α mRNA in TEC in Graves' disease
- 1. Heilongjiang Province Hospital, Haerbin (China). Dept. of Endocrinology
Description
To study the transcription profiles of interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) gene in human thyroid epithelial cells (TEC) from patients with Graves disease (GD), thyrocyte was isolated from thyroid tissues and cultured with RPMI-1640, then the expression of IL-10, TNF-α mRNA in the TEC was detected by means of RT-PCR. IL-10 mRNA expression in TEC was detected only in one case of GD group, and TNF-α mRNA was transcribed in all GD TEC. Neither IL-10 nor TNF-α mRNA was found in normal TEC. Thyroid as well as TEC can produce some kinds of cytokines. The transcription profiles of certain cytokines in the thyroid of patients with autoimmune thyroid disease (AITD) are different from those of normal thyroid tissues taken from MNG, which means that they are important features for the pathogenesis of AITD and abnormal thyroid function
Additional details
Publishing Information
- Journal Title
- Labeled Immunoassays and Clinical Medicine
- Journal Volume
- 10
- Journal Issue
- 2
- Journal Page Range
- p. 95, 99-101
- ISSN
- 1006-1703
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 35089618
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BLOOD SERUM; CELL CULTURES; DIAGNOSIS; EPITHELIUM; GENES; GOITER; HYPERTHYROIDISM; IMMUNITY; LYMPHOCYTES; LYMPHOKINES; MESSENGER-RNA; PATHOGENESIS; PATHOLOGY; POLYMERASE CHAIN REACTION; RESPONSE MODIFYING FACTORS; THYROID CELLS; TRANSCRIPTION
- Descriptors DEC
- ANIMAL CELLS; ANIMAL TISSUES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BLOOD PLASMA; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ENDOCRINE DISEASES; GENE AMPLIFICATION; GROWTH FACTORS; LEUKOCYTES; MATERIALS; MITOGENS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; RNA; SOMATIC CELLS