Published May 1993 | Version v1
Journal article Metadata-only

Two-site 'sandwich' immunoradiometric assay for thyrotropin with use of magnetizable particles

Creators

  • 1. Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy

Description

A two-site 'Sandwich' immunoradiometric assay for TSH with use of magnetizable particles is developed. The assay involves monoclonal antibody and polyclonal antibody. The monoclonal antibody is labeled with 1'25I. The polyclonal antibody is conjugated covalently to magnetizable particles. Sensitivity is 0.04 μIU/ml. Intra-assay coefficient of variation is 4.6-10.0%. Inter-assay coefficient of variation is 7.1-10.2%. Average recovery is more than 95%. The effects of assay procedure, incubation time and protein concentration on results are discussed

Additional details

Publishing Information

Journal Title
Journal of Isotopes
Journal Volume
6
Journal Issue
2
Journal Page Range
p. 65-70.
ISSN
1000-7512

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
25040022
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE; S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Descriptors DEI
BLOOD SERUM; IODINE 125; LABELLING; MONOCLONAL ANTIBODIES; RADIOIMMUNOASSAY; TSH
Descriptors DEC
ANTIBODIES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES