Analysis of differential proteome in rice anther with different heat tolerance based on iTRAQ technique

To reveal the molecular mechanism of heat tolerant and heat sensitive anther response to high temperature stress from protein level. The heat tolerance line 996 and heat sensitive line 4628 were chosen as research materials and the differential proteomics analysis was carried out on the rice anthers under high temperature and optimum temperature condition by using iTRAQ technique. The results showed that totally 957 differential expressed proteins were screened out in four comparable groups, 996 high temperature -996 optimal temperature, 4628 high temperature -4628 optimal temperature, 996 high temperature -4628 high temperature, 996 optimal temperature -4628 optimal temperature, among which 398 differential proteins were up-regulated, the proteins withover 20% coverage was 50.96% of the total protein number. GO analysis showed that the anther differentially expressed proteins in heading and flowering stage were mainly concentrated on metabolic processes, cell processes and other biological processes. The differentially expressed proteins were mainly distributed in intracellular parts, organelles and cells on cell composition and their molecular functions were majorly catalytic activity and binding. Analysis of anther differential protein pathway showed that 2 transcription factor (TF) genes belonging to bZIP and DOF family were differently expressed, the DOF family gene was up-related, and the bZIP family gene was down-regulated; majority of 18 HSPs genes were up-regulated. The genes related to plant hormone and signal transduction were mainly down-regulated. 5 genes of 10 differentially expressed genes related to reactive oxygen species (ROS) were differently expressed, 5 genes were up-regulated. The genes related to secondary metabolism were mainly down-regulated. The study can provide theoretical basis for revealing the mechanism of molecular regulation on rice anther response to heat stress. (authors)