Radiation induced enhancement of the efficiency of DNA mediated gene transfer in UV and x-ray sensitive CHO mutant cell lines
Creators
- 1. Medical Biophysics Unit, B.C. Cancer Research Centre, Vancouver, B.C. Canada V5Z 1L3
Description
The authors present results of experiments studying the enhancement effects of ionizing and non-ionizing radiation on the efficiency of DNA mediated gene transfer in mutant CHO cell lines. The established cell lines UV-5, UV-20 and EM9 are transfected with the recombinant DNA plasmid, pSV2-gpt irradiated with either UV or X-rays and plated in MAX selective media. MAX-resistant colonies are the result of the integration of pSV2-gpt and the expression of the gene coding for the bacterial xanthine-guanine phosphoribosyl transferase. Dose response curves for UV and X-rays are generated for the frequency of MAX-resistant colonies/survivor. From these experiments, the authors hope to delineate the role of DNA repair enzymes in the phenomenon at plasmid DNA integration after DNA mediated gene transfer
Additional details
Publishing Information
- Publisher
- Radiation Research Society.
- Imprint Place
- Philadelphia, PA (USA)
- Imprint Title
- Thirty-third annual meeting of the Radiation Research Society (Abstracts)
- Journal Page Range
- p. 40.
Conference
- Title
- 33. annual scientific meeting of the Radiation Research Society.
- Dates
- 5-9 May 1985.
- Place
- Los Angeles, CA (USA).
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 19049835
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; CHO CELLS; COLONY FORMATION; CULTURE MEDIA; DNA; DNA REPAIR; DOSE-RESPONSE RELATIONSHIPS; ENZYMES; GENETIC RADIATION EFFECTS; MUTAGENESIS; PLASMIDS; RADIATION INDUCED MUTANTS; RECOMBINANT DNA; STRUCTURE-ACTIVITY RELATIONSHI; SURVIVAL CURVES; ULTRAVIOLET RADIATION; X RADIATION
- Descriptors DEC
- ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CELL CONSTITUENTS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; IONIZING RADIATIONS; KINETICS; MUTANTS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; REACTION KINETICS; SOMATIC CELLS