Published October 1986
| Version v1
Journal article
Enzymatic method for the synthesis of [14C]pyridoxal 5-phosphate from [14C]pyridoxine
Description
A new enzymatic method for the synthesis of [14C]pyridoxal 5-phosphate is presented. [14C]Pyridoxal 5'-phosphate was synthesized from [14C]pyriodoxine through the successive actions of pyridoxal kinase and pyrdoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, [14C]pyridoxine, and both enzymes. [14C]Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of [14C]pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Volume
- 158
- Journal Issue
- 1
- Series
- Anal. Biochem.
- Journal Page Range
- 36-41
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18040917
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; BIOSYNTHESIS; CARBON 14 COMPOUNDS; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; LIVER; OXIDOREDUCTASES; PHOSPHATES; PHOSPHOTRANSFERASES; PYRIDOXAL; PYRIDOXINE; RATS; THIN-LAYER CHROMATOGRAPHY
- Descriptors DEC
- ALDEHYDES; ANIMALS; AZINES; BACTERIA; BODY; CARBON COMPOUNDS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; ENZYMES; GLANDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; KINETICS; MAMMALS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PYRIDINES; REACTION KINETICS; RODENTS; SEPARATION PROCESSES; SYNTHESIS; TRANSFERASES; VERTEBRATES; VITAMIN B GROUP; VITAMINS