Study the effect of gamma irradiation H2SO4, NaOH, and CH3COOH treatments for ethanol production from fermented rice straw

This experiment was carried out to know the ethanol and total carbohydrate concentrations in fermented rice straw which was irradiated with gamma ray 60-Co from latex irradiator with the doses of 0 and 200 kGy. Rice straw was mixed with H2SO4 NaOH 0,1 and 2% (v/v), (w/v) and with CH3COOH 0, 1, 3, and 5% (v/v) in the ratio 1:4 (w/v). The rice straw was crushed to be powder 6 mesh. Cellulase was added for saccharification. The yeast strain used for fermentation was S.Cerevisiae and the incubation time was 40 hours, at room temperature (28+/-2 Celsius centigrade). Results showed that gamma irradiation with dose of 200 kGy increased the ethanol and total carbohydrate productions (P=<0.01). Additions of H2SO4, NaOH also had significant effect to enchance the ethanol production (P=<0.05), (P=<0.01) but this effect was not significant on the total carbohydrate. Treatment with adding CH3COOH on rice straw had no significant effect either on ethanol and total carbohydrate of rice straw.The effect of interaction between irradiation and addition of solutions mentioned above had no significant effects, but the ethanol production had a tendency to increase with those combination treatments. (authors). 6 refs, 3 figs, 6 tabs