Influence of 103Pd radioactive stent on apoptosis of vascular smooth muscle cells
- 1. Sun Yat-sen Univ., Guangzhou (China). The Second Affiliated Hospital, Dept. of Cardiology
Description
Objective: To evaluate the influence of 103Pd radioactive stent on apoptosis and its relative genes bcl-2 and bax in injured vascular media smooth muscle cells of rabbit abdominal arteries and to investigate the mechanism of 103Pd radioactive stent for preventing restenosis after angioplasty. Methods: Fifty male New Zealand rabbits were randomized into stent group and 103Pd stent group. Each group was subdivided into 5 sub-groups. Control group was set up. The study arteries were harvested at 3, 7, 14, 28 and 56 d after stenting and the pathomorphology, apoptosis analysis and in situ hybridization were performed to evaluate the expression of bcl-2 and bax mRNA. Results: The severity of the restenosis in 103Pd stent group was less than that of stent group. It was most obvious at the 56th day (P<0.01). Using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method it was found that the 103Pd stent group had much more apoptosis of vascular smooth muscle cells than stent group did and reached the peak at the 7th day, (14.72±0.53)% vs (12.42±1.13)% (P<0.01). In situ hybridization analysis showed that the expression of bcl-2 mRNA of 103Pd stent group was much lower than that of stent group at 3 to 28 d. The difference was most obvious at the 28th day after stenting, (18.43± 0.67)% vs (21.55±0.93)% (P<0.01). The expression of bax mRNA of 103Pd stent group was higher than that of stent group, the peak was at the 7th day, (11.17±0.94)% vs (9.30±1.01)%. The ratio of bcl-2/bax in 103Pd stent group was much lower than that of stent group at 3 to 28 d. Linear correlation analysis showed that there was significant negative correlation between bcl-2 mRNA and apoptosis. Between bax mRNA and apoptosis, the positive correlation was found (P<0. 01). Conclusion: 103Pd radioactive stent induced more significant apoptosis in vascular media smooth muscle cells by promoting the expression of apoptosis related genes and relieved the expanding of restenosis
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Nuclear Medicine
- Journal Volume
- 23
- Journal Issue
- 5
- Journal Page Range
- p. 297-300
- ISSN
- 0253-9780
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 36021343
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- APOPTOSIS; BLOOD VESSELS; CORRELATION FUNCTIONS; DNA; EXPERIMENTAL DATA; GENE REGULATION; IN-SITU HYBRIDIZATION; LABELLING; MORPHOLOGY; MYOBLASTS; PALLADIUM 103; PATHOLOGY; PHOSPHOTRANSFERASES; PLASTIC SURGERY; RABBITS; RADIATION SOURCE IMPLANTS; RADIOTHERAPY; STATISTICS
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BIOTECHNOLOGY; BODY; CARDIOVASCULAR SYSTEM; DATA; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; EVEN-ODD NUCLEI; FUNCTIONS; GENETIC ENGINEERING; IMPLANTS; INFORMATION; INTERMEDIATE MASS NUCLEI; ISOTOPES; MAMMALS; MATHEMATICS; MEDICINE; MUSCLES; NUCLEAR MEDICINE; NUCLEI; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANS; PALLADIUM ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; RADIATION SOURCES; RADIOISOTOPES; RADIOLOGY; SURGERY; THERAPY; TRANSFERASES; VERTEBRATES