Published May 1977 | Version v1
Journal article Metadata-only

Radioassay of soluble immune complexes using their uptake by macrophage Fc receptors

Creators

  • 1. Debreceni Orvostudomanyi Egyetem (Hungary)

Description

Sera from pateints were tested for the presence of soluble immune complexes (IC) by a method based on the observation that IC inhibit uptake of aggregated IgG by the Fc receptors of macrophages. From the saturation curve of macrophages with 125I-labelled aggregates the optimal inhibitory effect of a measured amount of IC was determined. This radioassay is highly sensitive, being capable of detecting 20 ng of IC, is reproducible and covers a wide range of measurement (20 to 2000 ng). (author)

Additional details

Publishing Information

Journal Title
Immunology
Journal Volume
32
Journal Issue
5
Series
Immunology.
Journal Page Range
617-621

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
8324825
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACCURACY; ANTIGEN-ANTIBODY REACTIONS; IMMUNE SERUMS; IMMUNITY; IMMUNOGLOBULINS; IN VITRO; IODINE 125; MACROPHAGES; PERFORMANCE; RADIOASSAY; RADIOIMMUNOASSAY; SENSITIVITY; SPECIFICITY; UPTAKE
Descriptors DEC
ANIMAL CELLS; BETA DECAY RADIOISOTOPES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHAGOCYTES; PROTEINS; RADIOISOTOPES; SOMATIC CELLS; TRACER TECHNIQUES