Published July 11, 1985 | Version v1
Miscellaneous Restricted

Influence of the gas atmosphere on the effects of sparsely ionising rays on synchronised CHO cells as determined from survival rates and DNA strand breaks

Creators

Description

Oxygen saturation and anoxia were the two extremes chosen to test the effects of three different kinds of sparsely ionising radiation (15 MeV electrons giving relative depth doses of 30% and 100%; 200 kV X-radiation) on synchronised 4n CHO cells in different phases of the cell cycle. A reciprocal relation between strand break repair and cell survival was only observed for the influence of the gas atmosphere, while no such correlation could be established for the cell cycle and quality of rays as the other two parameters tested. This means that the extent of DNA damage still to be seen after strand break repair cannot be regarded as the sole measure of cell survival. (orig./MG)

Availability note (English)

MF available from INIS under the Report Number.

Abstract (German)

Synchronisierte 4n CHO-Zellen wurden in verschiedenen Phasen ihres Zellzyklus unter O2-Saettigung und Anoxie mit drei verschiedenen duenn ionisierenden Strahlenqualitaeten (15 MeV-Elektronen mit einer relativen Tiefendosis von 100% und 30%, 200 kV-Roentgenstrahlen) bestrahlt. Eine positive Korrelation zwischen Strangbruchreparatur und Zellueberleben konnte nur fuer den Einfluss der Gasatmosphaere nachgewiesen werden, nicht fuer den Einfluss von Zellzyklus und Strahlenqualitaet, d.h. das Ausmass der DNS-Schaedigung nach erfolgter Strangbruchreparatur kann nicht allein als Kriterium fuer das Zellueberleben angesehen werden. (orig./MG)

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Additional details

Additional titles

Original title (German)
Der Einfluss der Gasatmosphaere bei der Wirkung duenn ionisierender Strahlen auf synchronisierte CHO-Zellen gemessen an Ueberleben und DNS-Strangbruechen

Publishing Information

Imprint Pagination
138 p.
Report number
INIS-mf--12178

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
22031236
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Thesis
Descriptors DEI
ANOXIA; BIOLOGICAL REPAIR; CELL CULTURES; CELL CYCLE; CHO CELLS; ELECTRON BEAMS; EXTERNAL IRRADIATION; MEV RANGE 10-100; OXYGEN; RADIATION INJURIES; RADIATION QUALITY; STRAND BREAKS; SURVIVAL CURVES; X RADIATION
Descriptors DEC
ANIMAL CELLS; BEAMS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DISEASES; ELECTROMAGNETIC RADIATION; ELEMENTS; ENERGY RANGE; INJURIES; IONIZING RADIATIONS; IRRADIATION; LEPTON BEAMS; MEV RANGE; NONMETALS; PARTICLE BEAMS; RADIATION EFFECTS; RADIATIONS; SOMATIC CELLS