A modified technique for combination of cytochemistry and radioautography of haemic cells

Cultured mouse bone marrow and human blood cells were labelled with 3H-thymidine, smeared, and stained, using peroxydase reagents or the May-Gruenwald Giemsa procedure. The slides were dipped in an approximately 12% solution of polyvinylchloride, bleached by a photographic fixer and processed for liquid emulsion radioautography. This method yielded technically very satisfactory radioautographs of pre-stained smears. (author)