Published 1975 | Version v1
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The role of tryptophan in the photochemical crosslinking of peptides and proteins

Creators

  • 1. Commonwealth Scientific and Industrial Research Organization, Parkville (Australia). Div. of Protein Chemistry

Description

Irradiation of wool and several other proteins in the presence of 14C-labelled tryptophan results in the covalent binding of radioactive material to the protein. This result is consistent with the claim that irradiation of proteins introduces crosslinks involving tryptophan residues. Radiolabelling techniques have been used to study the products of irradiation of the tripeptide, Ala-14C-Trp-Val. Some of these products are polymeric - further evidence for the involvement of tryptophan in crosslinking reactions. Most of the yellow material produced during irradiation in air is polymeric. (orig.)

Availability note (English)

MF available from INIS under the Report Number.

Abstract (German)

Bei der Bestrahlung von Wolle und anderen Proteinen in Gegenwart von 14C-markiertem Tryptophan entsteht eine kavalente Bindung zwischen dem radioaktiven Material und dem Protein. Dieses Ergebnis bestaetigt die Behauptung, dass bei der Bestrahlung von Proteinen Querverbindungen mit Tryptophan-Rueckstaenden entstehen. Die Bestrahlungsprodukte des Tripeptids Ala-14C-Trp-Val wurden mit radioaktiven Markierungsmethoden untersucht. Einige dieser Produkte sind polymerisch - ein weiterer Beweis fuer die Rolle des Tryptophans in den Querverbindungsreaktionen. Der groesste Teil des bei der Bestrahlung an der Luft entstehenden gelben Materials ist polymerisch. (orig./AK)

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Additional details

Publishing Information

Imprint Pagination
10 p.
Report number
AED-Conf--75-388-003

Conference

Title
5. international symposium on wool textile research.
Dates
2 Sep 1975.
Place
Aachen, F.R. Germany.

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
7232748
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Resource subtype / Literary indicator
Conference
Descriptors DEI
CARBON 14 COMPOUNDS; CROSS-LINKING; IRRADIATION; LABELLING; PEPTIDES; PHOTOCHEMISTRY; PROTEINS; RADIOACTIVITY; TRYPTOPHAN; WOOL
Descriptors DEC
AMINO ACIDS; AZOLES; CARBON COMPOUNDS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CHEMISTRY; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INDOLES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYMERIZATION; PYRROLES

Optional Information

Notes
4 figs.; 1 tab.; 19 refs. With abstract.