Published January 2016 | Version v1
Journal article Metadata-only

Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants

Creators

  • 1. Department of Biology, East Carolina University, Greenville, NC 27858 (United States)
  • 2. Department of Biotechnology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054 (China)
  • 3. Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455 (United States)

Description

Highlights: • H2AX proteins seem to play a positive role in DNA DSB repair in Arabidopsis. • Genetic evidence is provided for diRNAs' roles in HR repair in Arabidopsis. • Genetic evidence is also provided for diRNAs' roles in NHEJ repair in Arabidopsis. • A model for H2AX and diRNA in DNA DSB repair in Arabidopsis is proposed. Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

Availability note (English)

Available from http://dx.doi.org/10.1016/j.mrfmmm.2015.12.002

Additional details

Identifiers

DOI
10.1016/j.mrfmmm.2015.12.002;
PII
S0027510715300531;

Publishing Information

Journal Title
Mutation Research
Journal Volume
783
Journal Page Range
p. 9-14
ISSN
0027-5107

INIS

Country of Publication
Netherlands
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
51084676
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ARABIDOPSIS; DNA; DNA REPAIR; GENE RECOMBINATION; GENETICS; HISTONES; NUCLEASES; RNA; STRAND BREAKS; ZINC
Descriptors DEC
BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BIOLOGY; DNA DAMAGES; ELEMENTS; ENZYMES; ESTERASES; HYDROLASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; PLANTS; PROTEINS; REPAIR

Optional Information

Notes
Published by Elsevier B.V.