Detection of HCMV infection of radiation workers with rapid-ELISA method

Objective: To establish a rapid method to detect HCMV with ELISA, and to study the infection conditions of radiation workers. Methods: using routine ELISA except adding 3% PEG to dilution solution to accelerate the reaction of antibody to antigen, the HCMV-IgG and HCMV-IgM in the serum of radiation workers were detected. Results: The total positive rate in 514 radiation workers in Jinan is 96.69%, of which 94.16% is latent infection, 0.19% is primary infection, 2.34% is relapse infection, and active infection rate is 2.53%. Conclusion: Radiation workers have certain risk to suffer from infection of HCMV which should delt with seriously. (authors)