Published January 2002
| Version v1
Journal article
1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins
Creators
- 1. University of Leeds, School of Biochemistry and Molecular Biology (United Kingdom)
- 2. Martek Biosciences (United States)
Description
The implementation of [13Cα,13C',15N,2Hα] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of 1JCα,Cβ couplings enables us to measure 1JCα,C' scalar and 1DCα,C' residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for 13C constant time experiments
Additional details
Identifiers
Publishing Information
- Journal Title
- Journal of Biomolecular NMR
- Journal Volume
- 22
- Journal Issue
- 1
- Journal Page Range
- p. 21-26
- ISSN
- 0925-2738
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 39109660
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AMINO ACIDS; CARBON 13; COUPLING CONSTANTS; COUPLINGS; HYDROGEN 1; LABELLING; NITROGEN 15; PROTEINS; SCALARS; SENSITIVITY
- Descriptors DEC
- CARBON ISOTOPES; CARBOXYLIC ACIDS; EVEN-ODD NUCLEI; HYDROGEN ISOTOPES; ISOTOPES; LIGHT NUCLEI; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; STABLE ISOTOPES
Optional Information
- Copyright
- Copyright (c) 2002 Kluwer Academic Publishers