Chromatographic and spectroscopic characterization of a red pigment obtained from Bothryodiplodia theobromae

A great variety of metabolites is obtained during the microbiological production of jasmonates from the fungus Bothryodiplodia theobromae. Many of them have not been characterized and can present interesting bioactive properties for their possible application. To achieve the identification, characterization and quantification of these metabolites constitutes a novel and complex analytic problem that is necessary to approach and that it can be solved using different chromatographic and spectroscopic methods. In this paper, the study of chromatographic and spectroscopic characterization of the resultant aqueous fraction obtained from the separation procedure by liquid-liquid extraction with solvents of different polarity of the broth of fermentation is shown. The aqueous fraction obtained was the most purified and was spectroscopically characterized by UV and to IR and chromatographically by TLC and HPLC. To define the chromatographic profile of this fraction, the chromatographic conditions were established by HPLC using an RP-18 column and this fraction was purified by column of Sephadex LH-20 and obtained a compound that suggests a structure similar to a flavonoid of the anthocyanin type