Biosensor applications of luminescence depolarization effects in protein-modified silica films doped with organic luminophores
Creators
- 1. Faculty of Mechanical Engineering, Wroclaw University of Technology, Wroclaw (Poland)
- 2. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw (Poland)
- 3. Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wroclaw (Poland)
- 4. Wroclaw University of Economics, Wroclaw (Poland)
- 5. Faculty of Mechanical Engineering, Wroclaw University of Technology, Wroclaw (Poland) and Electrotechnical Institute, Division of Electrotechnology and Materials Science, Wroclaw (Poland)
Description
Thin films of silica functionalized with NH2 groups were prepared by the sol-gel technique from tetraethoxysilane (TEOS) and (3-aminopropyl)-triethoxysilane (APTES) precursors. Selected proteins labeled with isothiocyanate fuorescein (FTIC) were covalently attached to their surfaces via the NH2 groups. The presence of the functional groups was confirmed by infrared absorption and Raman spectra measurements. The obtained films were immersed in a buffer and polarized emission spectra were measured. Next, the samples were incubated with selected bacterial endotoxin and the emission spectra were collected again. The influence of the endotoxin binding on the luminescence depolarization has been proven. The system based on this effect is suggested as an optical biosensor for the endotoxin recognition
Additional details
Identifiers
- DOI
- 10.1016/j.jlumin.2006.01.346;
- PII
- S0022-2313(06)00351-6;
Publishing Information
- Journal Title
- Journal of Luminescence
- Journal Volume
- 122-123
- Journal Page Range
- p. 987-989
- ISSN
- 0022-2313
- CODEN
- JLUMA8
Conference
- Title
- 2005 International conference on luminescence and optical spectroscopy of condensed matter
- Acronym
- ICL '05
- Dates
- 25-29 Jul 2005
- Place
- Beijing (China)
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 38037172
- Subject category
- S36: MATERIALS SCIENCE;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- DEPOLARIZATION; DOPED MATERIALS; EMISSION SPECTRA; ENDOTOXINS; ISOTHIOCYANATES; LUMINESCENCE; PROTEINS; RAMAN SPECTRA; SILICA; SOL-GEL PROCESS; THIN FILMS
- Descriptors DEC
- ANTIGENS; CARBONIC ACID DERIVATIVES; EMISSION; FILMS; HAZARDOUS MATERIALS; MATERIALS; MINERALS; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXIDE MINERALS; PHOTON EMISSION; SPECTRA; TOXIC MATERIALS; TOXINS
Optional Information
- Copyright
- Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.