Simultaneous determination of ascorbic acid, dopamine, and uric acid with polyaniline/hemin/reduced graphite oxide composite

Highlights: • PANI/H-rGO, as novel electrochemical sensor electrode, has been developed. • Ascorbic acid, dopamine, and uric acid were detected simultaneously. • Excellent sensitivity and selectivity were obtained. • High reproducibility and long storage stability were obtained. A novel and highly sensitive electrochemical sensor electrode, polyaniline/hemin/reduced graphite oxide, was synthesized. The electrode was then employed for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA), displayed good selectivity as well as excellent sensitivity. It showed low limit of detection of 0.05 µA/µM, 0.02 µA/µM, and 0.07 µA/µM in the simultaneous determination of AA, DA, and UA, respectively. Finally, high reproducibility (relative standard deviation, RSD = 2%, 1.5%, and 1% for AA, DA, and UA) and long storage stability (RSD = 3%, 1.2%, and 2% for AA, DA, and UA) of the electrode was observed.