Published November 1, 1989
| Version v1
Journal article
The derivative assay--an analysis of two fast components of DNA rejoining kinetics
Description
The DNA rejoining kinetics of human U-118 MG cells were studied after gamma-irradiation with 4 Gy. The analysis of the sealing rate of the induced DNA strand breaks was made with a modification of the DNA unwinding technique. The modification meant that rather than just monitoring the number of existing breaks at each time of analysis, the velocity, at which the rejoining process proceeded, was determined. Two apparent first-order components of single-strand break repair could be identified during the 25 min of analysis. The half-times for the two components were 1.9 and 16 min, respectively
Additional details
Publishing Information
- Journal Title
- Analytical Biochemistry
- Journal Volume
- 182
- Journal Issue
- 2
- Series
- Anal. Biochem.
- Journal Page Range
- 233-236
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21042750
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CATTLE; CHROMATOGRAPHY; DNA; DNA REPAIR; GAMMA RADIATION; GENETIC RADIATION EFFECTS; RADIATION EFFECTS; STRAND BREAKS; TUMOR CELLS
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; DOMESTIC ANIMALS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; IONIZING RADIATIONS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATIONS; RUMINANTS; SEPARATION PROCESSES; VERTEBRATES