Published October 21, 2004 | Version v1
Journal article Metadata-only

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Creators

  • 1. Centre for Biophotonics, SIBS, 27 Taylor Street, University of Strathclyde, Glasgow G4 0NR (United Kingdom)
  • 2. Department of Physics, John Anderson Building, University of Strathclyde, Glasgow G4 0NG (United Kingdom)

Description

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM

Availability note (English)

Available online at http://stacks.iop.org/0031-9155/49/4757/pmb4_20_007.pdf or at the Web site for the journal Physics in Medicine and Biology (ISSN 1361-6560) http://www.iop.org/

Additional details

Identifiers

URL
http://stacks.iop.org/0031-9155/49/4757/pmb4_20_007.pdf; http://www.iop.org/;
DOI
10.1088/0031-9155/49/20/007;
PII
S0031-9155(04)83135-X;

Publishing Information

Journal Title
Physics in Medicine and Biology
Journal Volume
49
Journal Issue
20
Journal Page Range
p. 4757-4763
ISSN
0031-9155
CODEN
PHMBA7

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
36032247
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
CALCIUM; CHO CELLS; FLUORESCENCE; LASERS; MICROSCOPY; PHOTONS; RATS; VEINS
Descriptors DEC
ALKALINE EARTH METALS; ANIMAL CELLS; ANIMALS; BLOOD VESSELS; BODY; BOSONS; CARDIOVASCULAR SYSTEM; ELEMENTARY PARTICLES; ELEMENTS; EMISSION; LUMINESCENCE; MAMMALS; MASSLESS PARTICLES; METALS; ORGANS; PHOTON EMISSION; RODENTS; SOMATIC CELLS; VERTEBRATES