Published January 1989 | Version v1
Journal article Metadata-only

Comparative studies of in vitro transformation by ethylene oxide and gamma-radiation of C3H/10T1/2 cells

Creators

  • 1. Stockholm Univ. (Sweden). Dept. of Radiobiology
  • 2. Stockholm Univ. (Sweden). Dept. of Mathematical Statistics
  • 3. Shell Research Ltd., Sittingbourne (UK). Milstead Lab. of Chemical Enzymology

Description

A dose-dependent transforming ability of the direct-acting alkylating agent, ethylene oxide (EtO), was demonstrated in C3H/10T1/2 mouse embryo fibroblasts. Morphologically transformed colonies were observed 5-6 weeks after the treatment with EtO. The transforming effectiveness of EtO was compared with that of gamma-radiation, and the rad-equivalence of EtO was calculated to be ∼ 90 rad/mMh. This value is consistent with earlier estimates based on induced mutation in various biological systems and thus indicates the applicability of the C3H/10T1/2 cell system in the risk estimation of human exposure to genotoxic compounds. (author)

Additional details

Publishing Information

Journal Title
Mutagenesis
Journal Volume
4
Journal Issue
1
Series
Mutagenesis.
Journal Page Range
58-61
ISSN
0267-8357
CODEN
MUTAE

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
20029278
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOASSAY; CELL TRANSFORMATIONS; COMPARATIVE EVALUATIONS; DOSE-RESPONSE RELATIONSHIPS; EMBRYOS; ETHYLENE; FIBROBLASTS; GAMMA RADIATION; IN VITRO; MICE; OXIDES
Descriptors DEC
ALKENES; ANIMAL CELLS; ANIMALS; CHALCOGENIDES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; EVALUATION; HYDROCARBONS; IONIZING RADIATIONS; MAMMALS; ONCOGENIC TRANSFORMATIONS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PERFORMANCE TESTING; RADIATIONS; RODENTS; SOMATIC CELLS; TESTING; VERTEBRATES