Evaluation of the effect of temperature, concentration and volumetric flow in the hydrolysis of sucrose by an immobilized invertase in a spherical reactor
- 1. Empresa Bridgstone Costa Rica (Costa Rica)
- 2. Univ. de Costa Rica, Escuela de Ingenieria Quimica (Costa Rica)
Description
The effect of the volumetric flow, the temperature and the initial concentration of sucrose in the reaction of hydrolysis of sucrose by immobilized invertase were evaluated in the laboratory. Invertase was immobilized in 20 g of support of mesh size between 120 and 140. The maximum quantity of immobilized invertase obtained has been 0,130 mg/g of support at 220 min. The first experimental stage has consisted in the evaluation of the effect of the initial concentration of sucrose (1,0 and 1,5 mol/L), the volumetric flow (3,0 mL/min and 4,0 mL/min) and the temperature (45 degrees C and 50 degrees C). The effect of the above three variable has been statistically significant. The conversion has been favorable for a concentration of sucrose 1,0 mol/L, a volumetric flow of 3 mL/min and a temperature of 50 degrees C. The maximum conversion obtained has been 95,4 %. The second experimental stage has analyzed the effect of the initial concentration of sucrose (0,75 and 1,0 mol/L), the volumetric flow (2,5 mL/min and 3,0 mL/min) and the temperature (50 degrees C and 55 degrees C). The variable of volumetric flow and the interaction concentration of sucrose - temperature are found statistically significant. The conversion has been favorable for a volumetric flow of 2,5 mL/min, and it has been preferable to work at a temperature of 50 degrees C with an initial concentration of sucrose of 1,0 mol/L. The maximum conversion has been 94,8 %. The effect of the concentration was analyzed in the last experimental stage, it was found that the maximum conversion percentage was 95,0 % for a concentration of 1,1 mol/L, for a temperature of 50 degrees C and for a volumetric flow of 2,5 mL/min. (author)
Availability note (English)
Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa RicaAbstract (Spanish)
El efecto del flujo volumetrico, la temperatura y la concentracion inicial de sacarosa, en la reaccion de hidrolisis de sacarosa utilizando una invertasa inmovilizada fueron evaluados a nivel de laboratorio. La invertasa fue inmovilizada en 20 g de soporte de tamano de malla entre 120 y 140. La cantidad maxima obtenida de enzima inmovilizada ha sido de 0,130 mg/g de soporte a los 220 min. La primera etapa experimental ha consistido en la evaluacion del efecto de la concentracion inicial de sacarosa (1,0 y 1,5 mol/L), el flujo volumetrico (3,0 mL/min) y la temperatura de reaccion (45 grados C y 50 grados C). El efecto de las tres variables anteriores ha sido estadisticamente significativo. La conversion se favorece para una concentracion 1,0 mol/L de sacarosa, un flujo volumetrico de 3 mL/min y una temperatura de 50 grados C. La maxima conversion obtenida ha sido de 95,4 %. La segunda etapa experimental ha evaluado el efecto de la concentracion inicial de sacarosa (0,75 y 1,0 mol/L), el flujo volumetrico (2,5 mL/min y 3,0 mL/min) y la temperatura (50 grados C y 55 grados C). La variable de flujo volumetrico y la interaccion concentracion de sacarosa - temperatura son encontradas significativas estadisticamente. La conversion se ha favorecido para un flujo de 2,5 mL/min, y se ha preferido operar a 50 grados C y a una concentracion 1,0 mol/L de sacarosa. El mayor porcentaje de conversion de sacarosa ha sido de un 94,8%. El efecto de la concentracion inicial de sacarosa se ha analizado en una ultima fase experimental, se ha encontrado que a una concentracion 1,1 mol/L es obtenido el mayor porcentaje de conversion de un 95,0%, para una temperatura de 50 grados C y un flujo volumetrico de 2,5 mL/min. (autor)Additional details
Additional titles
- Original title (Spanish)
- Evaluacion del efecto de la temperatura, concentracion y flujo volumetrico en la hidrolisis de sacarosa mediante una invertasa inmovilizada en un reactor esferico
Publishing Information
- Journal Title
- Ingenieria
- Journal Volume
- 21
- Journal Issue
- no.1
- Journal Page Range
- p. 61-74
- ISSN
- 1409-2441
INIS
- Country of Publication
- Costa Rica
- Country of Input or Organization
- Costa Rica
- INIS RN
- 45032388
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S60: APPLIED LIFE SCIENCES; S46: INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- EVALUATION; EXPERIMENTAL DATA; HYDROLYSIS; IMMOBILIZED CELLS; IMMOBILIZED ENZYMES; SACCHAROSE; SPECTROMETERS; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; TEMPERATURE CONTROL; VOLUMETRIC ANALYSIS
- Descriptors DEC
- CARBOHYDRATES; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; CONTROL; DATA; DECOMPOSITION; DISACCHARIDES; INFORMATION; LYSIS; MEASURING INSTRUMENTS; NUMERICAL DATA; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS; SACCHARIDES; SOLVOLYSIS
Optional Information
- Notes
- Figs., tabs., refs.