Mechanism of 2 Gy 60Co γ-ray-induced apoptosis in the regulatory T cells of mice
Creators
- 1. China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing (China)
Description
Objective: To analyze the influence of ionizing radiation on the viability of the regulatory T cells and the relationship between the expression of CD39 and apoptosis related factors in mice Treg cells. Methods: Mice were administered to whole body irradiation with 2 Gy of γ-rays, then lymphocytes were separated from thymus and spleen. The percentages of CD4+ T and CD4+ FOXP3+ CD25+ Treg lymphocytes, apoptosis related genes of Bax, Bcl-2 and CD39 were analyzed with FACS. Results: Treg cells had a higher survival fraction than CD4+FOXP3-Tcon cells after irradiation, and the time-responses of Treg cells in thymus and spleen were different. The apoptosis rate of Treg cells increased at 4 d after irradiation(t = -3.6, -7.4, P < 0.05). The Bax/Bcl-2 ratio and the CD39 expression were increased in thymus Treg cells (t = -3.4, -6.6, P < 0.05), but decreased in spleen Treg cells (t = 2.4, 2.6, P < 0.05). Conclusions: The changes of apoptosis and Bax/Bcl-2 are in consistent with the expression of CD39 in the irradiated Treg cells. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 33
- Journal Issue
- 4
- Journal Page Range
- p. 364-367
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 50013369
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- APOPTOSIS; COBALT 60; GAMMA RADIATION; GENES; GY RANGE 01-10; LYMPHOCYTES; MICE; SPLEEN; SURVIVAL CURVES; THYMUS; WHOLE-BODY IRRADIATION
- Descriptors DEC
- ABSORBED DOSE RANGE; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; GY RANGE; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; LYMPHATIC SYSTEM; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANS; RADIATION DOSE RANGES; RADIATIONS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 4 tabs., 9 refs.