Autoradiographic technic for Plutonium, Polonium, and Promethium tissue sections

Syrian hamster lung sections containing 238Pu, 239Pu, 210Po, or 147Pm are deparaffinized and hydrated to water. The slides are dipped in NTB emulsion, allowed to dry overnight, and exposed in Bakelite boxes at room temperature. They are then developed in D-19 for two to four minutes, rinsed in distilled water for ten seconds, fixed in Kodak fixer for five minutes, and washed at least 30 minutes in distilled water before staining with nuclear fast red and indigo-carmine. This autoradiographic technic, in conjunction with this particular staining procedure, gives a quick, efficient method for working with different α- and β-emitting radionuclides