Published September 1984
| Version v1
Journal article
Assays for detection of circulating IgG antibodies against Yellow Jacket Venom by radioimmunoprecipitation (RIP-IgG). Application to a immunotherapy submitted population
Description
Rapid, specific and reproducible radioimmunoprecipitation assay (RIP) for measuring IgG antibodies against Yellow Jacket Venom (YJV) has been developed. The YJV-I125 is added to serum sample and the immune complexes are precipitated by a second antibody with polyethylene glycol. IgG antibodies were measured by RIP in 24 patients receiving Yellow Jacket Venom immunotherapy. RIP assays were compared for 159 sera to a solid phase procedure (RAST IgG)
Abstract (French)
On a developpe une technique par radioimmunoprecipitation en phase liquide (RIP) pour le dosage des anticorps (Ac) IgG anti-venin de guepe. L'antigene (Ag) marque a l'iode 125 est incube avec le serum a tester; les complexes Ag-Ac sont ensuite precipites par un systeme deuxieme Ac-polyethylene glycol. La technique est rapide, specifique, et les coefficients de variation sont inferieurs a 8% en intra-essai et a 15% en inter-essai. On a suivi par cette methode les taux d'IgG specifiques chez 24 malades soumis a une desensibilisation au venin total de guepe. 159 serums ont ete testes en parallele par le RIP-IgG et une technique en phase solide (RAST-IgG Pharmacia)Additional details
Additional titles
- Original title (French)
- Dosage serique des IgG anti-venin de guepe par radioimmunoprecipitation (RIG-IgG). Application a des sujets en cours de desensibilisation
Publishing Information
- Journal Title
- Pathol.-Biol.
- Journal Volume
- 32
- Journal Issue
- 7
- Series
- Pathol.-Biol.
- Journal Page Range
- 785-790
- ISSN
- 0031-3009
INIS
- Country of Publication
- France
- Country of Input or Organization
- France
- INIS RN
- 16026933
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; IMMUNOGLOBULINS; IMMUNOTHERAPY; IODINE 125; LABELLED COMPOUNDS; RADIOIMMUNOASSAY; SPECIFICITY; VENOMS; WASPS
- Descriptors DEC
- ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; INSECTS; INTERMEDIATE MASS NUCLEI; INVERTEBRATES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; MEDICINE; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; THERAPY; TRACER TECHNIQUES