Published September 1997 | Version v1
Journal article Metadata-only

Effect of low-dose irradiation on expression of mRNA and protein. Pt.1. Induction of thioredoxin as radioprotective protein in human lymphocytes

Creators

  • 1. Central Research Inst. of Electric Power Industry, Abiko, Chiba (Japan). Abiko Research Lab.

Description

To elucidate the mechanism of hormetic effect by low-dose ionizing radiation, we studied the expression of the thioredoxin (TRX) gene in human lymphocytes after irradiation. TRX is a radioprotector and a key protein regulating cellular functions through redox reaction. The major results obtained were as follows; (1) The peaks of TRX mRNA expression and protein synthesis in human lymphocytes appeared 6-8 hr after irradiation with 25cGy. (2) At 6 hr after irradiation, the optimum dose for induction of TRX mRNA and TRX protein in human lymphocytes appeared to be 25-50cGy. (3) Induction of expression TRX mRNA had individual variations about twice. (4) Lymphocytes prepared from fresh venous blood showed the lowest TRX mRNA level in other cells such a Jurkat cells, lymphocytes stimulated for now with IL-2 and CD3 and the immortalized cell line 1G8. (5) The optimal dose and time course of induction of TRX by low-dose radiation suggest that TRX is related to the radio-adaptive response. (author)

Additional details

Publishing Information

Journal Title
Denryoku Chuo Kenkyusho Hokoku
Journal Issue
no.U97028
Journal Page Range
p. 1-4, 1-10
ISSN
1340-4652
CODEN
DCKHDL

INIS

Country of Publication
Japan
Country of Input or Organization
Japan
INIS RN
29059406
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
GAMMA RADIATION; GENETIC RADIATION EFFECTS; GENETICS; LOW DOSE IRRADIATION; LYMPHOCYTES; MESSENGER-RNA; POST-IRRADIATION EXAMINATION; PROTEINS; RADIATION DOSES; RADIOPROTECTIVE SUBSTANCES; TIME DEPENDENCE; X RADIATION
Descriptors DEC
ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BIOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DRUGS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; IONIZING RADIATIONS; IRRADIATION; LEUKOCYTES; MATERIALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RESPONSE MODIFYING FACTORS; RNA; SOMATIC CELLS