Study on the effects of gamma ray irradiation on the morphological variations of Brassia verrucosa Lindl. from in vitro bud clusters

The idea of creating sources of morphological variation of Warty Brassia (Brassia verrucosa Lindl.) was made on the basis of using in vitro bud clusters by gamma ray irradiation, aimed at selecting varieties to overcome these limitations and enhance the value of Warty Brassia. The study consists of the following contents: determining doses of gamma radiation to create variation of Warty Brassia; evaluating the effect of gamma radiation to the formation changes of the trees; isolated and selective variations to generate material source for mutation breeding. The results showed that gamma ray irradiation treatment, Co-60 source at doses 10-50 Gy of Warty Brassia from in vitro bud clusters isolated 6 different morphological variation; the variant of morphology, structure and diameter of pseudobulb were very difference comparing to control; the variant types are good growth and development in green house. In particular, there are two lines of variation, with large pseudobulb, dark green leaves and round pseudobulb, short leaves, is very promising for the development of potential mutants. The types of variation will be analyzed using molecular biology techniques to prove mutations and are going to continue selective color, flower shape in green house. (author)