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Published August 2020 | Version v1
Journal article Metadata-only

PIBF1 suppresses the ATR/CHK1 signaling pathway and promotes proliferation and motility of triple-negative breast cancer cells

Creators

  • 1. University of Ulsan College of Medicine, Asan Medical Center. Department of Radiation Oncology (Korea, Republic of)
  • 2. Korea Mouse Phenotyping Center (KMPC) (Korea, Republic of)
  • 3. University of Ulsan College of Medicine, Asan Medical Center. Department of Convergence Medicine (Korea, Republic of)
  • 4. Keimyung University. Department of Radiation Oncology (Korea, Republic of)
  • 5. University of Ulsan College of Medicine, Asan Medical Center. Division of Breast Surgery, Department of Surgery (Korea, Republic of)

Description

Purpose

: This study evaluates the oncogenic role of PIBF1 in triple-negative breast cancer (TNBC). TNBC is considered to have a poorer prognosis than other types of breast cancer and is associated with high risk of recurrence and distant metastasis. Currently, there are no effective therapies for the TNBC patients with distant metastasis due to the lack of targeted therapeutic options.

Methods

: The effects of PIBF1 knockdown on the cell viability and motility of TNBC cell lines were investigated. Effects of PIBF1 overexpression on tumorigenicity and cell motility were confirmed using Ba/F3 cell line and xenograft study on BALB/c nude mice.

Results

: In TNBC cell lines that highly express PIBF1, knockdown of PIBF1 induces apoptosis and suppresses cell viability and motility with activation of the ATR/CHK1 signaling pathway. Moreover, the oncogenic function of PIBF1 was confirmed using the Ba/F3 cell line.

Conclusion

: For the first time, these findings clarify the role of PIBF1 in regulating ATR/CHK1 signaling pathway and inhibiting the proliferation and migration of TNBC cell lines. These results demonstrate the oncogenic roles of PIBF1 and provide new insights into the function and the molecular mechanism of PIBF1 in malignant TNBC.

Additional details

Identifiers

DOI
10.1007/s10549-020-05732-0;

Publishing Information

Journal Title
Breast Cancer Research and Treatment
Journal Volume
182
Journal Issue
3
Journal Page Range
p. 591-600
ISSN
0167-6806
CODEN
BCTRD6

INIS

Country of Publication
Netherlands
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
55060590
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANGIOGENESIS; APOPTOSIS; CELL CULTURES; CELL PROLIFERATION; GTP-ASES; HAZARDS; MAMMARY GLANDS; METASTASES; MICE; MIGRATION; NEOPLASMS; PATIENTS; SIGNALS; THERAPY; TUMOR CELLS; VIABILITY
Descriptors DEC
ACID ANHYDRASES; ANIMAL CELLS; ANIMALS; BODY; DISEASES; ENZYMES; GLANDS; HYDROLASES; MAMMALS; MEDICINE; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; VERTEBRATES

Optional Information

Copyright
Copyright (c) 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020