Ex-situ Generation of the Gold Nanowire Networks Bovine Serum Albumin Bio-Conjugated System Using Pulsed Laser Ablation in a Harsh Environment

The experimental conditions were investigated to fabricate gold nanowire networks (AuNNWs) by pulsed laser ablation in saline solutions. The study revealed that the production of alternative nanoparticles (or aggregates) or nanowire networks at the fundamental wavelength of a Nd:YAG laser (1064 nm) depend on the medium media surrounding Au nanoparticles during the process of synthesis. The aggregates and precipitates of the gold nanoparticles (AuNPs) in saline solutions were caused by the harsh environment of the anions with Na+ cations in the solution. The ex-situ was used for the preparation of bovine serum albumin (BSA) bio-conjugates. Enhanced colloidal stability was dramatically achieved when BSA was adsorbed onto Au nanowire networks. The optical extinction spectra of the colloids in the UV/vis region were obtained to evaluate the structure of the dispersed Au phase. Transmission electron microscopy (TEM) was applied to visualize the size and morphology of the colloidal particles. Au nanowire networks compatibility can be prepared and applied in real environments. (paper)