Effects of trichostatin a on the expression of sodium/iodide symporter mRNA and the uptake of iodide in human thyroid cancer cell lines

Objective: To investigate the sodium/iodide symporter (NIS) expression and iodide uptake in thyroid cancer cells induced by the histone deacetyltransferase inhibitors (HDACi), Trichostatin A (TSA). Methods: Both the thyroid cancer cell lines, follicular thyroid carcinoma cell line FTC-133 and papillary thyroid carcinoma cell line K1, were firstly induced with TSA for 48 h. Then, the expression of NIS mRNA was analysed with reverse transcription-polymerase chain reaction (RT-PCR), the densitometric ratio of NIS/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was calculated, and the iodide uptake in the thyroid cancer cells was also measured. Independent-sample t-test and one-way analysis of variance (ANOVA) were used to analyze the data. Results: For FTC-133 cells, increased NIS mRNA expression was detected after 48 h of TSA treatment, and the changes were dose-dependent (F=32.56, P<0.01).No obvious increasing expression of NIS mRNA was observed in K1 cells after 48 h of TSA treatment (F=2.823, P >0.05). Furthermore, FTC-133 cells showed the ability of accumulating radioiodide with 50 and 75 nmol/L TSA induction for 48 h: (15.42 ± 0.42) x 103 counts · min-1 · 10-5 cells vs (8.46 ± 0.84) x 103 counts · min-1 · 10-5 cells, t=3.018, P<0.05; (18.98 ± 1.33) x 103 counts · min-1 · 10-5 cells vs (8.46 ± 0.84) x 103 counts · min-1 · 10-5 cells, t=3.557, P<0.05, respectively, while radioiodide uptake in K1 displayed no significant change after TSA induction: (5.83 ± 1.09) x 103 counts · min-1 · 10-5 cells, (6.97 ± 0.65) x 103 counts · min-1 · 10-5 cells vs (5.37 ± 0.88) x 103 counts · min-1 · 10-5 cells, t=0.185, P> 0.05 and t = 0.332, P > 0.05, respectively. Conclusion: TSA induced upregulated NIS mRNA expression in follicular thyroid cancer cells and augmented radioiodide uptake in thyroid cancer cells, while TSA had no remarkable effect on papillary thyroid carcinoma cell. (authors)