Development of identification process for insect group using radiation marker DNA
Creators
- 1. National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)
Description
Detection of a band pattern for insect groups was tried by using radiation marked DNA clone. A rapid segregation process for poly-type DNA segment was investigated. A band pattern of silkworm was detected by analysis using DNA type transposon, K1.4. The exon regions on genes of hemiptera insect were segregated by in vitro cloning. Band patterns of the silkworm and the other insects were detected by identification process of DNA clone and radiation marker. Family singularity mutation existed in the inserted position of transposon. The family of insect was identified easily by the difference of the detection band patterns. Effective band pattern for family discrimination were obtained by analysis for a part of mitochondria DNA and ribosomal DNA. DNA segregation process was investigated by using the enriched library, also. (M. Suetake)
Additional details
Publishing Information
- Journal Title
- Kokuritsu Kikan Genshiryoku Shiken Kenkyu Seika Hokoku-Sho
- Journal Issue
- no.43
- Journal Page Range
- p. 99.1-99.4
- ISSN
- 0288-8874
INIS
- Country of Publication
- Japan
- Country of Input or Organization
- Japan
- INIS RN
- 36039801
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BANDING TECHNIQUES; BIOLOGICAL MARKERS; DNA HYBRIDIZATION; DNA SEQUENCING; DNA-CLONING; EXONS; IN VITRO; INSECTS; INTRONS; SILKWORM; TRANSPOSONS
- Descriptors DEC
- ANIMALS; ARTHROPODS; BIOTECHNOLOGY; CLONING; CYTOLOGICAL TECHNIQUES; DNA HYBRIDIZATION; GENETIC ENGINEERING; HYBRIDIZATION; INSECTS; INVERTEBRATES; LEPIDOPTERA; MOTHS; NUCLEIC ACID HYBRIDIZATION; STRUCTURAL CHEMICAL ANALYSIS
Optional Information
- Notes
- 12 refs., 3 figs., 2 tabs.